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KMID : 0545119980080060606
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Journal of Microbiology and Biotechnology
1998 Volume.8 No. 6 p.606 ~ p.612
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Production of Biopolymer Flocculant by Bacillus subtilis TB11
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Yoon, Sang Hong
Song, Jae Kyeung/Go, Seung Joo/Ryu, Jin Chang
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Abstract
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A microbial flocculant-producing gram-positive bacterium, strain TB11, was isolated from soil samples, and was identified as Bacillus subtilis by using the Midi system, the Biolog system, 16S rDNA sequence analysis, and some physiological and morphological characteristics. The maximum flocculant capsular biopolymer of TB11 strain (BCP, 4.9 ㎎/㎖) was obtained when it was grown in GA broth medium containing 3% glutamic acid, 2% glycerol, 0.5% citric acid, 0.5% NH_4Cl, 0.05% MgSO_4·7H_2O, 0.05% K_2HPO_4 and 0.004% FeCl_3. 6H_2O, pH 7.2, at 30℃ for 70 h with shaking. When glycerol was used as an additional carbon source in the GA medium, TB11 produced only flocculant BCP without any by-product. The flocculant (BCP) was found to aggregate suspended kaolin and activated charcoal powder without cations, and its flocculating activity was significantly enhanced by the addition of bivalent cations such as Ca^2+, Zn^2+, and Mn^2+. The flocculation activity by addition of Ca^2+ was high in an acidic pH 4.0. In the case of Zn^2+, high flocculating activity remained without significant loss in the broad range of pH 4.0 to 9.0.
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